A novel drug-free strategy of nano-pulse stimulation sequence (NPSS) in oral cancer therapy: In vitro and in vivo study

作者：Guo, Jinsong; Dong, Feihong; Ding, Lian; Wang, Kaile; Zhang, Jue^*; Fang, Jing

来源：Bioelectrochemistry, 2018, 123: 26-33.

DOI：10.1016/j.bioelechem.2018.04.010

• Nano-pulse stimulation sequence (NPSS)
• Oral cancer
• NO
• ROS
• Ca2+

Nano-pulse stimulation (NPS) is a novel technology to induce cancer apoptosis. In this study, based on the energy-dose effect of NPS, we designed a special NPS sequence (NPSS) with low field intensity. The effectiveness and mechanisms of NPSS on oral cancer therapy were evaluated by cell proliferation assays, microscopic investigation, JC-1 mitochondrial membrane potential assay, tumor inhibition assays, immunohistochemistry (IHC) assay, Ca2+, NOS and ROS detection assays, respectively. The results demonstrated that NPSS treatment significandy inhibited oral cancer growth in vitro and in vivo. Furthermore, we found that NPSS treatment induced an obviously apoptosis and mitochondrial membrane potential (A Pm) reduction in Cal-27 cells. Notably, further experiments revealed that the mechanisms of crosstalk signaling between NO, ROS and Ca2+ involvement in NPSS treatment. In conclusion, this is a proof-of-concept study that provides a potential alternative strategy for developing and applying NPSS in oral cancer therapy.

• 出版日期2018-10
• 单位北京大学