Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes

作者：Copeland Matthew F; Politz Mark C; Pfleger Brian F^*

来源：Current Opinion in Biotechnology, 2014, 29: 46-54.

DOI：10.1016/j.copbio.2014.02.010

The last several years have witnessed an explosion in the understanding and use of novel, versatile trans-acting elements. TALEs, CRISPR/Cas, and sRNAs can be easily fashioned to bind any specific sequence of DNA (TALEs, CRISPR/Cas) or RNA (sRNAs) because of the simple rules governing their interactions with nucleic acids. This unique property enables these tools to repress the expression of genes at the transcriptional or post-transcriptional levels, respectively, without prior manipulation of cis-acting and/or chromosomal target DNA sequences. These tools are now being harnessed by synthetic biologists, particularly those in the eukaryotic community, for genome-wide regulation, editing, or epigenetic studies. Here we discuss the exciting opportunities for using TALEs, CRISPR/Cas, and sRNAs as synthetic trans-acting regulators in prokaryotes.

• 出版日期2014-10