Aflatoxin B-1 and M-1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators

作者：Loi Martina; Fanelli Francesca; Zucca Paolo; Liuzzi Vania C; Quintieri Laura; Cimmarusti Maria T; Monaci Linda; Haidukowski Miriam; Logrieco Antonio F; Sanjust Enrico; Mule Giuseppina^*

来源：Toxins, 2016, 8(9): 245.

DOI：10.3390/toxins8090245

• laccase
• Pleurotus
• mycotoxins
• aflatoxin B-1
• aflatoxin M-1
• biodegradation
• redox mediators

Laccases (LCs) are multicopper oxidases that find application as versatile biocatalysts for the green bioremediation of environmental pollutants and xenobiotics. In this study we elucidate the degrading activity of Lac2 pure enzyme form Pleurotus pulmonarius towards aflatoxin B-1 (AFB(1)) and M-1 (AFM(1)). LC enzyme was purified using three chromatographic steps and identified as Lac2 through zymogram and LC-MS/MS. The degradation assays were performed in vitro at 25 degrees C for 72 h in buffer solution. AFB(1) degradation by Lac2 direct oxidation was 23%. Toxin degradation was also investigated in the presence of three redox mediators, (2,2-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]) (ABTS) and two naturally-occurring phenols, acetosyringone (AS) and syringaldehyde (SA). The direct effect of the enzyme and the mediated action of Lac2 with redox mediators univocally proved the correlation between Lac2 activity and aflatoxins degradation. The degradation of AFB(1) was enhanced by the addition of all mediators at 10 mM, with AS being the most effective (90% of degradation). AFM(1) was completely degraded by Lac2 with all mediators at 10 mM. The novelty of this study relies on the identification of a pure enzyme as capable of degrading AFB(1) and, for the first time, AFM(1), and on the evidence that the mechanism of an effective degradation occurs via the mediation of natural phenolic compounds. These results opened new perspective for Lac2 application in the food and feed supply chains as a biotransforming agent of AFB(1) and AFM(1).

• 出版日期2016-9