Monitoring the HIV-1 integrase enzymatic activity using atomic force microscopy in a 2LTR system

作者：Guy Shlomit; Rotem Dvir; Hayouka Zvi; Gabizon Ronen; Levin Aviad; Zemel Limor; Loyter Abraham; Porath Danny^*; Friedler Assaf

来源：Chemical Communications, 2013, 49(30): 3113-3115.

DOI：10.1039/c3cc40748a

Integration of the HIV cDNA into the host chromosome is a key event in the viral replication cycle. It is mediated by the viral integrase (IN) enzyme, which is an attractive anti-HIV drug target. Here we present the first AFM imaging of IN-mediated DNA integration products in a two-LTR system.

• 出版日期2013