A plant-derived quadrivalent virus like particle influenza vaccine induces cross-reactive antibody and T cell response in healthy adults

作者：Pillet Stephan; Aubin Eric; Trepanier Sonia; Bussiere Diane; Dargis Michele; Poulin Jean Francois; Yassine Diab Bader; Ward Brian J; Landry Nathalie

来源：Clinical Immunology, 2016, 168: 72-87.

DOI：10.1016/j.clim.2016.03.008

• Plant-derived quadrivalent influenza vaccine
• Virus-like particles
• Nicotiana benthamiana
• Safety and immunogenicity
• Cell-mediated immunity
• Cross-reactive response

Recent issues regarding efficacy of influenza vaccines have re-emphasized the need of new approaches to face this major public health issue. In a phase 1-2 clinical trial, healthy adults received one intramuscular dose of a seasonal influenza plant-based quadrivalent virus-like particle (QVLP) vaccine or placebo. The hemagglutination inhibition (HI) titers met all the European licensure criteria for the type A influenza strains at the 3 mu g/strain dose and for all four strains at the higher dosages 21 days after immunization. High HI titers were maintained for most of the strains 6 months after vaccination. QVLP vaccine induced a substantial and sustained increase of hemagglutinin-specific polyfunctional CD4 T cells, mainly transitional memory and T-EMRA effector IFN-gamma(+) CD4 T cells. A T cells cross-reactive response was also observed against A/Hong-Kong/1/1968 H3N2 and B/Massachusetts/2/2012. Plant-based QVLP offers an attractive alternative manufacturing method for producing effective and HA-strain matching seasonal influenza vaccines.

• 出版日期2016-7