A Study of the Metabolome of Ricinus communis for Forensic Applications

作者：Ovenden Simon P B^*; Gordon Benjamin R; Bagas Christina K; Muir Bob; Rochfort Simone; Bourne David J

来源：Australian Journal of Chemistry, 2010, 63(1): 8-21.

DOI：10.1071/CH09293

Investigations were undertaken to ascertain the appropriateness of studying the metabolome of Ricinus communis for cultivar and provenance determination. Seeds from 14 R. communis specimens (a total of 56 seeds) collected from the east coast of Australia were analyzed by high pressure liquid chromatography with UV detection (HPLC-UV), liquid chromatography-mass spectrometry (LC-MS), and (1)H NMR spectroscopy. The collected data were then analyzed using principle component analysis (PCA). For HPLC-UV analysis, six R. communis specimens were unambiguously identified by PCA as belonging to separate classes relating to specimen. LC-MS data allowed unique ions to be identified for four specimens. Conversely 10 specimens were unambiguously segregated in the PCA of the (1)H NMR data. The ratio of ricinine 1 to demethylricinine analogues 2 and 3 was found to be important for specimen determination. These combined analyses suggested that a combination of HPLC-UV and (1)H NMR in conjunction with PCA could allow for specimen differentiation.

• 出版日期2010