An optimized isotopic labelling strategy of isoleucine-gamma(2) methyl groups for solution NMR studies of high molecular weight proteins

作者：Ayala Isabel; Hamelin Olivier; Amero Carlos; Pessey Ombeline; Plevin Michael J; Gans Pierre; Boi**ouvier Jerome^*

来源：Chemical Communications, 2012, 48(10): 1434-1436.

DOI：10.1039/c1cc12932e

An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-gamma(2) groups in proteins. The H-2, C-13-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.

• 出版日期2012
• 单位中国地震局