A Calorimetric Study on the Interaction of Zinc and Cadmium Ions with Jack Bean Urease

作者：Behbehani Rezaei G; Saboury A A; Sabbaghy F

来源：Chinese Journal of Chemistry, 2011, 29(3): 446-450.

• jack bean urease
• isothermal titration calorimetry
• inhibitor
• zinc

A thermodynamic study on the interaction of Jack bean urease, JBU, with Zn(2+) and Cd(2+) ions was studied by isothermal titration calorimetry (ITC) at 290, 300 and 310 K in 30 mmol/L Tris buffer solution, pH 7.0. The heats of JBU+Zn(2+) and JBU+Cd(2+) interactions are reported and analyzed in terms of the extended solvation theory. It was indicated that there is a set of 12 identical and non-interacting binding sites for Zn(2+) and Cd(2+) ions. The interactions of Zn(2+) and Cd(2+) ions with JBU are exothermic and both enthalpy and entropy driven. The association equilibrium constants for JBU Zn2+ complexes are 4118.20, 3354.70 and 2790.62 L.mol(-1) at 290, 300 and 310 K respectively. The association equilibrium constants for JBU+Cd(2+) interactions are 2831.6 and 2386.28 L.mol(-1) at 300 and 310K, respectively.

• 出版日期2011-3