DEPENDENCE OF PHENANTHRENE DISSIPATION IN MANGROVE SEDIMENT ON THE DISTANCE TO ROOT SURFACE OF KANDELIA OBOVATA L

作者：Fang Yu; Yan Chong Ling^*; Du Jing Na; Yu Jun Yi

来源：International Journal of Phytoremediation, 2012, 14(6): 596-608.

DOI：10.1080/15226514.2011.616240

• polycyclic aromatic hydrocarbons (PAHs)
• rhizoremediation
• mangrove plant
• denaturing gradient gel electrophoresis (DGGE)
• sediment enzymes

We explored the relationship between the distance from roots and rhizosphere-mediated phenanthrene (PHE) dissipation in planted mangrove sediment. A compartmentalized device (rhizobox) was used that separated root surface and four consecutive rhizosphere layers that extended 0-2, 2-4, 4-6, and >6 mm away from the root surface. Kandelia obovata L. Druce seedlings were grown for 4 months in sediment spiked with 10 mg.kg(-1) PHE. PHE dissipation, microbial community structure and enzymatic activities at millimeter scale in the vicinity of plant roots were analyzed. Results indicated significant differences in PHE dissipation through the various layers in the planted rhizobox. PHE dissipation exhibited the most rapid loss in the 0-2 mm near-rhizosphere layer, the lowest in far-rhizosphere (>6 mm) layer. Microbial community structure as indicated by denaturing gradient gel electrophoresis (DGGE) profiles showed that special bacterial species and larger bacterial numbers were observed in near-rhizosphere layers. Depending on the distance to root surface, near-rhizosphere bacterial species and enzymatic activities were more abundant than root compartment and far-rhizosphere, resulting in rapid dissipation of PHE.

• 出版日期2012
• 单位厦门大学