Binding of several benzodiazepines to bovine serum albumin: Fluorescence study

作者：Machicote Roberta G; Pacheco Maria E; Bruzzone Liliana^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2010, 77(2): 466-472.

DOI：10.1016/j.saa.2010.06.020

• Bovine serum albumin
• Benzodiazepines
• Fluorescence quenching
• Fluorescence resonance energy transfer
• Thermodynamic parameters

The interactions of lorazepam, oxazepam and bromazepam with bovine serum albumin (BSA) were studied by fluorescence spectrometry. The Stern-Volmer quenching constants and corresponding thermodynamic parameters Delta H, Delta G and Delta S were calculated. The binding constants and the number of binding sites were also investigated. The distances between the donor (BSA) and the acceptors (benzodiazepines) were obtained according to fluorescence resonance energy transfer and conformational changes of BSA were observed from synchronous fluorescence spectra.

• 出版日期2010-10-1