Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline

作者：Gulcin Ilhami^*; Elias Riad; Gepdiremen Akcahan; Chea Aun; Topal Fevzi

来源：Journal of Enzyme Inhibition and Medicinal Chemistry, 2010, 25(1): 44-53.

DOI：10.3109/14756360902932792

• Antioxidant activity
• cepharanthine
• fangchinoline
• Stephania rotunda
• alkaloid
• radical scavenging

In the present study, we determined the antioxidant activity of cepharanthine and fangchinoline from Stephania rotunda by performing different in vitro antioxidant assays, including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging, superoxide anion (O-2(center dot-)) radical scavenging, hydrogen peroxide scavenging, total antioxidant activity, reducing power, and ferrous ion (Fe2+) chelating activities. Cepharanthine and fangchinoline showed 94.6 and 93.3% inhibition on lipid peroxidation of linoleic acid emulsion at 30 mu g/mL concentration, respectively. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, and trolox indicated inhibitions of 83.3, 92.2, 72.4, and 81.3% on peroxidation of linoleic acid emulsion at the same concentration (30 mu g/mL), respectively. According to the results, cepharanthine and fangchinoline have effective antioxidant and radical scavenging activity.

• 出版日期2010-2