Molecular phylogenetic diversity of the emerging mucoralean fungus Apophysomyces: Proposal of three new species

作者：Alvarez Eduardo; Stchigel Alberto M; Cano Josep^*; Sutton Deanna A; Fothergill Annette W; Chander Jagdish; Salas Valentina; Rinaldi Michael G; Guarro Josep

来源：Revista Iberoamericana de Micologia, 2010, 27(2): 80-89.

DOI：10.1016/j.riam.2010.01.006

• Apophysomyces elegans
• Apophysomyces ossiformis
• Apophysomyces trapeziformis
• Apophysomyces variabilis
• Phylogeny
• Taxonomy
• Zygomycetes

Background: Apophysomyces is a monotypic genus belonging to the order Mucorales. The species Apophysomyces elegans has been reported to cause severe infections in immunocompromised and immunocompetent people. In a previous study of Alvarez et al.(3) [J Clin Microbiol 2009;47:1650-6], we demonstrated a high variability among the 5.8S rRNA gene sequences of clinical strains of A. elegans.
Material and methods: We performed a polyphasic study based on the analysis of the sequences of the histone 3 gene, the internal transcribed spacer region of the rDNA gene, and domains D1 and D2 of the 28S rRNA gene, as well as by evaluation of some relevant morphological and physiological characteristics of a set of clinical and environmental strains of A. elegans.
Results and conclusions: We have demonstrated that A. elegans is a complex of species. We propose as new species Apophysmyces ossiformis, characterised by bone-shaped sporangiospores, Apophysomyces trapeziformis, with trapezoid-shaped sporangiospores, and Apophysomyces variabilis, with variable-shaped sporangiospores. These species failed to assimilate esculin, whereas A. elegans was able to assimilate that glycoside. Amphotericin B and posaconazole are the most active in vitro drugs against Apophysomyces.

• 出版日期2010-6-30