A Comparison of Recombinant Receptor-Reporter Gene Bioassays and a Total Estrogen Enzyme Linked Immunosorbent Assay for the Rapid Screening of Estrogenic Activity in Natural and Waste Waters

作者：Allinson Mayumi; Shiraishi Fujio; Allinson Graeme^*

来源：Bulletin of Environmental Contamination and Toxicology, 2011, 86(5): 461-464.

DOI：10.1007/s00128-011-0251-x

• Estrogenic activity
• Recombinant receptor-reporter gene bioassay
• Total estrogens ELISA

In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hER alpha bioassay (r(2) = 0.93), but not for the medER alpha bioassay (r(2) = 0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurments were very good (r(2) > 0.95). There was no correlation between bioassay response and ELISA ES measurments for estuarine samples.

• 出版日期2011-5