Determination of Eucomic Acid in Chinese Hawthorn Fruit and Products by Gas Chromatography-Mass Spectrometry

作者：Gao, Zhe; Wang, Rong-Fang; Kang, Xiao-Hu; Qin, Ai-Xia; Jia, Ya-Nan; He, Tong-Sen; Pan, Yu-Lei; Cui, Tong^*

来源：Asian Journal of Chemistry, 2014, 26(16): 5053-5057.

DOI：10.14233/ajchem.2014.16312

• Eucomic acid
• Eucomic acid 1,4-dimethyl ester
• GC-MS
• Crataegus pinnatifida
• Flavor component

An analytical method for determination of eucomic acid in Chinese hawthorn fruit and its products by gas chromatography-mass spectrometry (GC-MS) was developed. The sample was extracted by ethanol, esterificated with sulphuric acid-methanol solution and then extracted with dichloromethane. The final analysis was performed by GC-MS using the fragment ions m/z 107 under selected ion monitoring (SIM) for the content of eucomic acid. External standard method was used for quantification. Under optimum conditions, there was a good linearity within the range of 2-500 mu g/g. The limit of detection was 0.35 mu g/g, the recoveries were in the range 87-102 %, the relative standard deviation (RSD) was 3.1-5.6 %. This method was applied to determine the eucomic acid in hawthorn fruit and its products. The content is between 5.1-255 mu g/g.

• 出版日期2014-8
• 单位河北农业大学