Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations

作者：Szirmay Balazs; Kustan Peter; Horvath Szalai Zoltan; Ludany Andrea; Lakatos Agnes; Muhl Diana; Wittmann Istvan; Miseta Attila; Kovacs Gabor L; Koszegi Tamas^*

来源：Bioanalysis, 2018, 10(6): 377-384.

DOI：10.4155/bio-2017-0228

• acute kidney injury
• cystatin-C
• tubular damage
• turbidimetry
• urine
• validation

Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

• 出版日期2018-3