A combination of GDNF and hUCMSC transplantation loaded on SF/AGs composite scaffolds for spinal cord injury repair

作者：Jiao, Genlong; Lou, Guofeng; Mo, Yunfei; Pan, Yongqin; Zhang, Ziyong; Guo, Rui^*; Li, Zhizhong^*

来源：Materials Science & Engineering C-Materials for Biological Applications, 2017, 74: 230-237.

DOI：10.1016/j.msec.2016.12.017

• Alginate microspheres
• Silk fibroin
• Scaffold
• Glial cell-derived neurotrophic factor
• Human umbilical cord mesenchymal stem cells
• Spinal cord injury

Spinal cord injury (SCI) is a severe trauma for which no effective treatment is currently available. In this study, a composited treatment system was prepared using a silk fibroin/alginates/glial cell line-derived neurotrophic factor (SF/AGs/GDNF) scaffold seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) and the combined therapeutic effects of the composite scaffold to repair SCI rats were evaluated. The use of SF as a scaffold material could act as a biomimetic platform allowing neurons to properly accommodate and rebuild the target tissue. The SF/AGs/GDNF scaffold had the best sustained-release function and the AGs were the key determining factor in the controlled release of GDNF. After 8 weeks of treatment, the hUCMSCs on SF/AGs/GDNF composite scaffolds could significantly enhance the scar expansion of spinal cord tissue and increased the number of surviving neurons. The combination of GDNF and hUCMSCs transplantation loaded on SF/AGs composite scaffolds exhibited better therapeutic and repair effects to the SCI of rats, compared with the SF/AGs group or GDNF alone on SF/AGs scaffolds. The composite scaffold, GDNF and stem cells could build a bioactive material to form the micro-environment of growth and repair of the neurons. These results may provide a theoretical basis and beneficial exploration for clinical treatment of SCI.

• 出版日期2017-5-1
• 单位暨南大学