Monitoring bioreactors using principal component analysis: production of penicillin G acylase as a case study

作者：Nucci Edson Romano; Cruz Antonio J G; Giordano Roberto C^*

来源：Bioprocess and Biosystems Engineering, 2010, 33(5): 557-564.

DOI：10.1007/s00449-009-0377-y

• Multivariate statistical process control (MSPC)
• Fault detection
• Bacillus megaterium
• Bioreactor monitoring
• PCA

The complexity of biological processes often makes impractical the development of detailed, structured phenomenological models of the cultivation of microorganisms in bioreactors. In this context, data pre-treatment techniques are useful for bioprocess control and fault detection. Among them, principal component analysis (PCA) plays an important role. This work presents a case study of the application of this technique during real experiments, where the enzyme penicillin G acylase (PGA) was produced by Bacillus megaterium ATCC 14945. PGA hydrolyzes penicillin G to yield 6-aminopenicilanic acid (6-APA) and phenyl acetic acid. 6-APA is used to produce semi-synthetic beta-lactam antibiotics. A static PCA algorithm was implemented for on-line detection of deviations from the desired process behavior. The experiments were carried out in a 2-L bioreactor. Hotteling's T (2) was the discrimination criterion employed in this multivariable problem and the method showed a high sensibility for fault detection in all real cases that were studied.

• 出版日期2010-6