Antibodies to HIV-1 gp41 recognize synthetic peptides of human IFN-alpha and IFN-beta

作者：Bai Y; Zhao YX; Yu TW; Dierich MP; Chen YH^*

来源：International Archives of Allergy and Immunology, 2000, 121(2): 170-172.

DOI：10.1159/000024313

• HIV-1 gp41
• IFN-alpha
• IFN-beta
• common epitope

Based on our finding that a common epitope exists between HIV-1 gp41 and human type I interferons (IFN-alpha and IFN-beta), and increased levels of antibodies against human IFN-alpha and IFN-beta were observed in HIV-1-infected individuals, we tried to explain the mechanism of increased levels of antibodies. Mouse antisera recognizing HIV-1 recombinant soluble (rs) gp41 (aa 539-684) interacted with two synthetic peptides sequence-corresponding to the IFN-alpha/beta receptor binding site on human IFN-alpha and IFN-beta, white normal mouse serum (pooled normal sera) did nor. The anti-rspg41 antisera after adsorption by IFN-beta sepharose column lost the activity of interaction with both synthetic peptides. In another experiment, rsgp4? could bind to sepharose column conjugated with anti-IFN-beta polyclonal antibodies (IgG). These results indicate that the common epitope on gp41 and type 1 interferons could induce antibodies recognizing the receptor binding site on IFN-alpha and IFN-beta, suggesting that increased levels of antibodies against IFN-alpha and IFN-beta in HIV-1-infected individuals could be induced by gp41.

• 出版日期2000-2
• 单位清华大学