No acute toxicity to Uca pugnax, the mud fiddler crab, following a 96-h exposure to sediment-bound permethrin

作者：Stueckle Todd A^*; Griffin Kristin; Foran Christy M

来源：Environmental Toxicology, 2008, 23(4): 530-538.

DOI：10.1002/tox.20363

• pyrethroids
• permethrin
• osmoregulation
• glutathione S-transferase
• Uca pugnax

In coastal areas, the application of pyrethroid insecticides and the resulting sediment residues pose a potential threat to marine benthic ecosystems. Pyrethroids cause acute toxicity and exhibit a wide range of sublethal effects on fish and crustaceans when exposure is aqueous. Fiddler crabs that inhabit salt marsh sediment are sensitive to sediment-associated pollutants and serve as a sentinel species for xenobiotic exposure. We exposed adult U. pugnax to salt marsh sediment spiked with different 60% trans/40% cis permethrin concentrations for 96 h, and evaluated changes in oxygen consumption rate, hemolymph osmolarity, and glutathione S-transferase activity (GST) following exposure. Marsh sediment was not lethal to U. pugnax at permethrin concentrations of 100-10,000 mu g/kg. Sediment-bound permethrin had no significant effect on respiration and osmoregulation. Exposure caused an induction of hepatopancreas GST in a dose-dependent manner. Gill and midgut tissues showed induction at permethrin concentrations at 10,000 mu g/kg. We conclude that short term exposure to permethrin-contaminated sediment does not pose a significant threat to this species or impact respiration and osmoregulation. Furthermore, increased GST activity allows us to evaluate this enzyme's induction as a generalist biomarker for sediment-bound pyrethroid exposures.

• 出版日期2008-8