Denitrogenation of carbazole by a novel strain Bacillus Clausii BS1 isolated from Egyptian Coke

作者：Zakaria Basem S; Nassar Hussein N; EL Gendy Nour Sh^*; El Temtamy Seham A; Sherif S Mourad

来源：Energy Sources, Part A: Recovery, Utilization, and Environmental Effects , 2016, 38(13): 1840-1851.

DOI：10.1080/15567036.2015.1004389

• Bacteria
• biodenitrogenation pathway
• carbazole
• identification
• isolation

In this study, an effective enrichment technique was applied to isolate different bacterial strains with capabilities to denitrogenate carbazole (CAR), as a model compound for nitrogenous polyaromatic hydrocarbons (NPAHs). Twenty-four different CAR-denitrogenating bacterial strains were isolated from mineral coke samples. From all, a Gram +ve bacterial isolate designated BS1 showed a higher biodenitrogenation (BDN) efficiency relevant to the well-known biodenitrogenating bacterium strain Pseudomonas resinovorans CA10, recording 77.15 and 60.66% removal of 1000 ppm CAR with the production of 119.79 and 102.43 ppm anthranilic acid, and 121.19 and 90.33 ppm catechol, as by-products, respectively. BS1 was identified by 16S rDNA gene sequence analysis to be Bacillus clausii BS1 (NCBI GenBank Accession no. KF985981) with a similarity of 99.14%. The CAR-BDN pathway was also elucidated using high-performance liquid chromatography (HPLC) and gas chromatography mass spectrometry analysis (GC/MS), which proved further metabolism of catechol to muconic acid.

• 出版日期2016