Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

作者：Al Rubaye Ali^*; Nabok Alexei; Catanante Gaelle; Marty Jean Louis; Takacs Ezster; Szekacs Andras

来源：Sensors and Actuators B: Chemical , 2018, 263: 248-251.

DOI：10.1016/j.snb.2018.02.220

• Aptamer
• Ochratoxin A
• Label-free optical biosensor
• Total internal reflection ellipsometry
• Binding kinetics

The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label -free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KD =1.810(-8) Mol which is characteristic for highly specific aptamer/OTA binding.

• 出版日期2018-6-15