A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease

作者：Sanchez Lopez E; Happe H; Steenvoorden E; Crego A L; Marina M L; Peters D J M; Mayboroda O A^*

来源：Molecular Biosystems, 2017, 13(10): 1940-1945.

DOI：10.1039/c7mb00245a

Metabolic profiling provides an unbiased view of the physiological status of an organism as a "function'' of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 mu m-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).

• 出版日期2017-10-1