A single centrifugation method for isolating fat droplets from cells and tissues

作者：Harris Lydia Ann L S; Shew Trevor M; Skinner James R; Wolins Nathan E^*

来源：The Journal of Lipid Research, 2012, 53(5): 1021-1025.

DOI：10.1194/jlr.D023598

• adipose tissue
• fatty acid/metabolism
• lipid droplets
• lipids
• liver
• brown adipose tissue
• cell fractionation
• organelle isolation

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge.jlr This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.-Harris, L-A. L. S., T. M. Shew, J. R. Skinner, and N. E. Wolins. A single centrifugation method for isolating fat droplets from cells and tissues. J. Lipid Res. 2012. 53: 1021-1025.

• 出版日期2012-5