ACTIVATION OF MICROBUBBLES BY SHORT-PULSED ULTRASOUND ENHANCES THE CYTOTOXIC EFFECT OF CIS-DIAMMINEDICHLOROPLATINUM (II) IN A CANINE THYROID ADENOCARCINOMA CELL LINE IN VITRO

作者：Sasaki Noboru; Kudo Nobuki; Nakamura Kensuke; Lim Sue Yee; Murakami Masahiro; Kumara W R Bandula; Tamura Yu; Ohta Hiroshi; Yamasaki Masahiro; Takiguchi Mitsuyoshi^*

来源：Ultrasound in Medicine and Biology, 2012, 38(1): 109-118.

DOI：10.1016/j.ultrasmedbio.2011.09.017

• Diagnostic ultrasound
• Ultrasound targeted microbubble destruction
• Drug delivery

Ultrasound targeted microbubble destruction has succeeded in delivering drugs and genes. This study was designed to explore characteristics of ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. Canine thyroid adenocarcinoma cells were exposed to short-pulsed diagnostic ultrasound in the presence of cis-diamminedichloroplatinum (II) (cisplatin) and ultrasound contrast agent Sonazoid (R) microbubbles. The cytotoxic effect of cisplatin was enhanced by short-pulsed diagnostic ultrasound and microbubbles. Incubation time with microbubbles influenced the cytotoxic effect of cisplatin. However, exposure duration did not affect the cytotoxic effect of cisplatin. Therefore, short-pulsed diagnostic ultrasound may activate microbubbles near cells and deliver cisplatin into cells. In addition, activation of microbubbles may be concluded in a short time. Our results suggest that short exposure duration could be potentially sufficient to induce efficient drug delivery by ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. (E-mail: [e-mail].

• 出版日期2012-1