Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion

作者：Gyongyosi Mariann^*; Posa Aniko; Pavo Noemi; Hemet**erger Rayyan; Kvakan Heda; Steiner Boker Sabine; Zsolt Petrasi; Ferenc Manczur; Pavo Imre J; Edes Istvan F; Wojta Johann; Glogar Dietmar; Huber Kurt

来源：Thrombosis and Haemostasis, 2010, 104(2): 376-384.

DOI：10.1160/TH09-08-0558

• Stem cell mobilisation
• haematopoietic
• mesenchymal
• stromal-derived
• ischaemic preconditioning

Effects of ischaemic preconditioning (IP) on the mobilisation and recruitment of haematopoietic (HSCs) and mesenchymal stem (MSC) cells were determined in porcine coronary occlusion/reperfusion. Thirty-three pigs underwent percutaneous occlusion of the left anterior descending coronary artery (LAD) for 90 minutes (min), followed by 120 min reperfusion. IP was performed in 16 of the 33 pigs by two cycles of 5 min balloon occlusion/reperfusion prior to the 90 min occlusion (group IP vs. group C). Peripheral blood and myocardial tissue concentration of bone marrow origin HSCs (characterised by coexpression of CD31+, CD90+, CD45+) and MSCs (characterised by coexpression of CD44+, CD90+, CD45-) were measured by flow cytometry in the early phase of P. Plasma/serum levels of stem cell mobilisation factors (stromal cell-derived factor-1 alpha [SDF-1 alpha], vascular endothelial growth factor [VEGF], tumour necrosis factor alpha[TNF-alpha] and interleukin-8 [IL-8]) were measured. IP led to a significant increase in circulating HSCs as compared with the group C (475 +/- 233 vs. 281 +/- 264/mu l, p=0.032) in the early phase of IP. In contrast, a rapid and prolonged decrease in level of circulating MSCs was observed in group IP as compared with group C (19 +/- 12 vs. 32 +/- 17/mu l, p=0.015). The recruitment of HSCs and MSCs in infarct and border zone was significantly greater in IP group, indicating a faster homing of MSCs as compared with the rate of mobilisation. Rapid increase in VEGF, TNF-alpha and IL-8 levels was induced by IP, which, however, was not correlated with the levels of circulating SCs. In conclusion, IP resulted in differential mobilisation and recruitment of HSCs and MSCs in the early phase of cardioprotection.

• 出版日期2010-8