Design of a novel gut bacterial adhesion model for probiotic applications

作者：Rodes Laetitia; Coussa Charley Michael; Marinescu Daniel; Paul Arghya; Fakhoury Marc; Abbasi Sana; Khan Afshan; Tomaro Duchesneau Catherine; Prakash Satya^*

来源：Artificial Cells Nanomedicine and Biotechnology, 2013, 41(2): 116-124.

DOI：10.3109/10731199.2012.712047

• adherence
• bacteria
• biofilm
• bioreactor
• gut microbiota
• gut model
• mucosa
• probiotics

A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin-alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.

• 出版日期2013-4