A Comparative Study of Nitrilases Identified by Genome Mining

作者：Kaplan Ondrej; Vesela Alicja B; Petrickova Alena; Pasquarelli Fabrizia; Picmanova Martina; Rinagelova Anna; Bhalla Tek Chand; Patek Miroslav; Martinkova Ludmila^*

来源：Molecular Biotechnology, 2013, 54(3): 996-1003.

DOI：10.1007/s12033-013-9656-6

• Nitrilase
• Arylacetonitrilase
• Cyanide hydratase
• Substrate specificity
• Nitrile
• Cyanide

Escherichia coli strains expressing different nitrilases transformed nitriles or KCN. Six nitrilases (from Aspergillus niger (2), A. oryzae, Neurospora crassa, Arthroderma benhamiae, and Nectria haematococca) were arylacetonitrilases, two enzymes (from A. niger and Penicillium chrysogenum) were cyanide hydratases and the others (from P. chrysogenum, P. marneffei, Gibberella moniliformis, Meyerozyma guilliermondi, Rhodococcus rhodochrous, and R. ruber) preferred (hetero)aromatic nitriles as substrates. Promising nitrilases for the transformation of industrially important substrates were found: the nitrilase from R. ruber for 3-cyanopyridine, 4-cyanopyridine and bromoxynil, the nitrilases from N. crassa and A. niger for (R,S)-mandelonitrile, and the cyanide hydratase from A. niger for KCN and 2-cyanopyridine.

• 出版日期2013-7