A development and an improvement of selectable markers in Pleurotus ostreatus transformation

作者：Matsunaga Yukari; Ando Megumi; Izumitsu Kosuke; Suzuki Kazumi; Honda Yoichi; Irie Toshikazu

来源：Journal of Microbiological Methods, 2017, 134: 27-29.

DOI：10.1016/j.mimet.2017.01.007

• Mushroom
• White-rot
• Basidiomycete

Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3 +/- 0.6 transformants/mu g plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7 +/- 11.5 transformants/mu g plasmid DNA.

• 出版日期2017-3