Aubergine is a component of a nanos mRNA localization complex

作者：Becalska Agata N; Kim YoungJung R; Belletier Nicolette G; Lerit Dorothy A; Sinsimer Kristina S; Gavis Elizabeth R^*

来源：Developmental Biology, 2011, 349(1): 46-52.

DOI：10.1016/j.ydbio.2010.10.002

• Aubergine
• nanos
• Drosophila
• mRNA localization
• Rumplestiltskin
• Segmentation

Localization of nanos (nos) mRNA to the posterior pole of the Drosophila oocyte is essential for abdominal segmentation and germline development during embryogenesis. Posterior localization is mediated by a complex cis-acting localization signal in the nos 3' untranslated region that comprises multiple partially redundant elements. Genetic analysis suggests that this signal is recognized by RNA-binding proteins and associated factors that package nos mRNA into a localization competent ribonucleoprotein complex. However, functional redundancy among localization elements has made the identification of individual localization factors difficult. Indeed, only a single direct-acting nos localization factor, Rumpelstiltskin (Rump), has been identified thus far. Through a sensitized genetic screen, we have now identified the Argonaute family member Aubergine (Aub) as a nos localization factor. Aub interacts with nos mRNA in vivo and co-purifies with Rump in an RNA-dependent manner. Our results support a role for Aub, independent of its function in RNA silencing, as a component of a nos mRNA localization complex.

• 出版日期2011-1-1