Morphofunctional modifications in gill mitochondria-rich cells of Mozambique tilapia transferred from freshwater to 70% seawater, detected by dual observations of whole-mount immunocytochemistry and scanning electron microscopy

作者：Choi Jeong Hyun^*; Lee Kyung Mi; Inokuchi Mayu; Kaneko Toyoji

来源：Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 2011, 158(1): 132-142.

DOI：10.1016/j.cbpa.2010.09.019

• Mitochondria-rich cell
• Mozambique tilapia
• Na+/K+-ATPase
• Na+/Cl- cotransporter
• Na+/K+/2Cl(-) cotransporter
• Na+/H+ exchanger-3

Acute responses of gill mitochondria-rich (MR) cells to direct transfer from freshwater to 70% seawater were examined in a euryhaline teleost Mozambique tilapia (Oreochromis mossambicus). Scanning electron microscopic (SEM) observations revealed that apical openings of MR cells were morphologically classified into an apical pit, a convex apical surface, a concave apical surface, and a transitory apical surface. Meanwhile, in whole-mount immunocytochemistry with anti-Na+/K+-ATPase (NKA), T4 antibody (detecting apical Na+/Cl- cotransporter (NCC) and basolateral Na+/K+/2Cl(-) cotransporter (NKCC)), and anti-Na+/H+ exchanger-3 (NHE3), NKA-immunoreactive MR cells were functionally classified into immature cells without both NKCC/NCC and NHE3 (type I), ion-absorptive cells with apical NCC (type II), those with apical NHE3 (type III), and ion-secretory cells with basolateral NKCC (type IV). Dual observations of whole-mount immunocytochemistry and SEM clearly showed morphofunctional alterations in MR cells. After transfer to 70% seawater, type-II MR cells with a convex surface or pit closed their apical openings to suspend ion absorption. Type-III MR cells with a concave surface or pit were transformed into type-IV MR cells with an enlarged pit, via a transitory surface. Our findings indicate functional plasticity of type-III/IV MR cells to switch ion-transport functions, whereas type-II MR cells are considered to be specific for freshwater adaptation.

• 出版日期2011-1