Analysis for Apoptosis and Necrosis on Adipocytes, Stromal Vascular Fraction, and Adipose-Derived Stem Cells in Human Lipoaspirates after Liposuction

作者：Wang Wei Z^*; Fang Xin Hua; Williams Shelley J; Stephenson Linda L; Baynosa Richard C; Wong Nancy; Khiabani Kayvan T; Zamboni William A

来源：Plastic and Reconstructive Surgery, 2013, 131(1): 77E-85E.

DOI：10.1097/PRS.0b013e3182729ff7

Background: Adipose-derived stem cells have become the most studied adult stem cells. The authors examined the apoptosis and necrosis rates for adipocyte, stromal vascular fraction, and adipose-derived stem cells in fresh human lipoaspirates.
Methods: Human lipoaspirate (n = 8) was harvested using a standard liposuction technique. Stromal vascular fraction cells were separated from adipocytes and cultured to obtain purified adipose-derived stem cells. A panel of stem cell markers was used to identify the surface phenotypes of cultured adipose-derived stem cells. Three distinct stem cell subpopulations (CD90(+)/CD45(-), CD105(+)/CD45(-), and CD34(+)/CD31(-)) were selected from the stromal vascular fraction. Apoptosis and necrosis were determined by annexin V/propidium iodide assay and analyzed by flow cytometry.
Results: The cultured adipose-derived stem cells demonstrated long-term proliferation and differentiation evidenced by cell doubling time and positive staining with oil red O and alkaline phosphatase. Isolated from lipoaspirates, adipocytes exhibited 19.7 +/- 3.7 percent apoptosis and 1.1 +/- 0.3 percent necrosis; stromal vascular fraction cells revealed 22.0 +/- 6.3 percent of apoptosis and 11.2 +/- 1.9 percent of necrosis; stromal vascular fraction cells had a higher rate of necrosis than adipocytes (p < 0.05). Among the stromal vascular fraction cells, 51.1 +/- 3.7 percent expressed CD90(+)/CD45(-), 7.5 +/- 1.0 percent expressed CD105(+)/CD45(-), and 26.4 +/- 3.8 percent expressed CD34(+)/CD31(-). CD34(+)/CD31(-) adipose-derived stem cells had lower rates of apoptosis and necrosis compared with CD105(+)/CD45(-) adipose-derived stem cells (p < 0.05).
Conclusions: Adipose-derived stem cells had a higher rate of apoptosis and necrosis than adipocytes. However, the extent of apoptosis and necrosis was significantly different among adipose-derived stem cell subpopulations. (Plast. Reconstr. Surg. 131: 77e, 2013.)

• 出版日期2013-1