DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL

作者：Rodrigues de Menezes Francisca Gleire; Neves Soraya da Silva; de Sousa Oscarina Viana; Vieira Maia Vila Nova Candida Machado; Maggioni Rodrigo; Diogo Theophilo Grace Nazareth; Hofer Ernesto; Silva dos Fernandes Vieira Regine Helena

来源：Revista do Instituto de Medicina Tropical de Sao Paulo, 2014, 56(5): 427-432.

DOI：10.1590/S0036-46652014000500010

• Cholera
• Estuaries
• Pathogenicity
• Genes

The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes.

• 出版日期2014-10