A novel chimeric amine dehydrogenase shows altered substrate specificity compared to its parent enzymes

作者:Bommarius Bettina R; Schuermann Martin; Bommarius Andreas S*
来源:Chemical Communications, 2014, 50(95): 14953-14955.
DOI:10.1039/c4cc06527a

摘要

We created a novel chimeric amine dehydrogenase (AmDH) via domain shuffling of two parent AmDHs ('L- and F-AmDH'), which in turn had been generated from leucine and phenylalanine DH, respectively. Unlike the parent proteins, the chimeric AmDH ('cFL-AmDH') catalyzes the amination of acetophenone to (R)-methylbenzylamine and adamantylmethylketoneto adamantylethylamine.

  • 出版日期2014