B1a lymphocytes in the rectal mucosa of ulcerative colitis patients

作者：Polese Lino^*; Boetto Riccardo; De Franchis Giuseppe; Angriman Imerio; Porzionato Andrea; Norberto Lorenzo; Sturniolo Giacomo Carlo; Macchi Veronica; De Caro Raffaele; Merigliano Stefano

来源：World Journal of Gastroenterology, 2012, 18(2): 144-149.

DOI：10.3748/wjg.v18.i2.144

• B1 cell
• CD5
• Flow cytometry
• Rectum
• Ulcerative colitis

AIM: To assess B1a cell expression in the rectal mucosa of ulcerative colitis (UC) patients in comparison with healthy controls. %26lt;br%26gt;METHODS: Rectal mucosa biopsies were collected from 15 UC patients and 17 healthy controls. CD5(+) B cells were analysed by three colour flow cytometry from rectal mucosal samples after mechanical disaggregation by Medimachine (R). Immunohistochemical analysis of B and T lymphocytes was also performed. Correlations between, on the one hand, rectal B1a cell concentrations and, on the other, erythrocyte sedimentation rate and C-reactive protein levels and clinical, endoscopic and histological disease activity indices were evaluated. %26lt;br%26gt;RESULTS: Rectal B-lymphocyte (CD19(+)/CD45(+)) rate and concentration were higher in UC patients compared with those in healthy controls (47.85% +/- 3.12% vs 26.10% +/- 3.40%, P = 0.001 and 501 +/- 91 cells/mm2 vs 117 +/- 18 cells/mm2, P %26lt; 0.001); Rectal B1a cell density (CD5(+)CD19(+)) was higher in UC patients than in healthy controls (85 +/- 15 cells/mm2 vs 31 +/- 6.7 cells/mm2, P = 0.009). Rectal B1a cell (CD5/CD19(+)) rate correlated inversely with endoscopic classification (Rs = -0.637, P %26lt; 0.05). %26lt;br%26gt;CONCLUSION: B1a lymphocytes seem to be involved in the pathogenesis of UC, however, the role they play in its early phases and in disease activity, have yet to be defined.

• 出版日期2012-1-14