Actinopolyspora righensis sp nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria

作者：Meklat Atika; Bouras Noureddine; Zitouni Abdelghani; Mathieu Florence; Lebrihi Ahmed; Schumann Peter; Sproeer Cathrin; Klenk Hans Peter; Sabaou Nasserdine^*

来源：Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 2013, 104(3): 301-307.

DOI：10.1007/s10482-013-9948-7

• Actinopolyspora righensis sp nov halophilic actinomycete
• Algerian Sahara
• Polyphasic taxonomy

A novel halophilic actinomycete strain, H23(T), was isolated from a Saharan soil sample collected in DjamA cent a (Oued Righ region), El-Oued province, South Algeria. Strain H23(T) was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23(T) had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136(T)) to 94.8 % (Actinopolyspora mortivallis DSM 44261(T)). The strain grew optimally at pH 6.0-7.0, 28-32 A degrees C and in the presence of 15-25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23(T) had MK-10 (H-4) and MK-9 (H-4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C-17:0 (37.4 %), iso-C-17:0 (14.8 %), iso-C-15:0 (14.2 %), and iso-C-16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23(T) (=DSM 45501(T) = CCUG 63368(T) = MTCC 11562(T)).

• 出版日期2013-9