Low alpha(2)beta(1) Integrin Function Enhances the Proliferation of Fibroblasts from Patients with Idiopathic Pulmonary Fibrosis by Activation of the beta-Catenin Pathway

作者：Xia Hong; Seeman Jeremy; Hong Jian; Hergert Polla; Bodem Vidya; Jessurun Jose; Smith Karen; Nho Richard; Kahm Judy; Gaillard Philippe; Henke Craig^*

来源：American Journal Of Pathology, 2012, 181(1): 222-233.

DOI：10.1016/j.ajpath.2012.03.034

Idiopathic pulmonary fibrosis (IPF) is a progressive and incurable fibroproliferative disorder characterized by unrelenting proliferation of fibroblasts and their deposition of collagen within alveoli, resulting in permanently scarred, nonfunctional airspaces. Normally, polymerized collagen suppresses fibroblast proliferation and serves as a physiological restraint to limit fibroproliferation after tissue injury. The IPF fibroblast, however, is a pathologically altered cell that has acquired the capacity to elude the proliferation-suppressive effects of polymerized collagen. The mechanism for this phenomenon remains incompletely understood. Here, we demonstrate that expression of alpha(2)beta(1) integrin, a major collagen receptor, is pathologically low in IPF fibroblasts interacting with polymerized collagen. Low integrin expression in IPF fibroblasts is associated with a failure to induce PP2A phosphatase activity, resulting in abnormally high levels of phosphorylated (inactive) GSK-3 beta and high levels of active beta-catenin in the nucleus. Knockdown of beta-catenin in IPF fibroblasts inhibits their ability to proliferate on collagen. Interdiction of alpha(2)beta(1) integrin in control fibroblasts reproduces the IPF phenotype and leads to the inability of these cells to activate PP2A, resulting in high levels of phosphorylated GSK-3 beta and active beta-catenin and in enhanced proliferation on collagen. Our findings indicate that the IPF fibroblast phenotype is characterized by low alpha(2)beta(1) integrin expression, resulting in a failure of integrin to activate PP2A phosphatase, which permits inappropriate activation of the beta-catenin pathway. (Am J Pathol 2012, 181:222-233; http://dx.doi.org/10.1016/j.ajpath.2012.03.034)

• 出版日期2012-7
• 单位南京医科大学