Development of SSR Markers Linked to Low Hydrocyanic Acid Content in Sorghum-Sudan Grass Hybrid Based on BSA Method

作者：Yu Xiao-Xia; Liu Zhi-Hua; Yu Zhuo^*; Shi Yue; Li Xiao-Yu

来源：Protein and Peptide Letters, 2016, 23(5): 417-423.

DOI：10.2174/0929866523666160322153559

• Bulked segregant analysis
• Hydrocyanic acid
• Single marker analysis
• Sorghum-Sudan grass hybrid
• SSR markers

Sorghum-Sudan grass hybrid containing high hydrocyanic acid content can cause hydrocyanic acid poisoning to the livestock and limit the popularization of this forage crop. Molecular markers associated with low hydrocyanic acid content can speed up the process of identification of genotypes with low hydrocyanic acid content. In the present study, 11 polymorphic SSR primers were screened and used for bulked segregant analysis and single marker analysis. Three SSR markers Xtxp7(230), Xtxp7(375) and Bnlg667(960) associated with low hydrocyanic acid content were rapidly identified by BSA. In single marker analysis, six markers Xtxp7(230), Xtxp7(375), Bnlg667(960), Xtxp67-11, Xtxp295-7 and Xtxp12-9 were linked to low hydrocyanic acid content, which explained the proportion of phenotypic variation from 7.6 % to 41.2 %. The markers identified by BSA were also verified by single marker analysis. The three SSR marker bands were then cloned and sequenced for sequence homology analysis in NCBI. It is the first report on the development of molecular markers associated with low hydrocyanic acid content in sorghum-Sudan grass hybrid. These markers will be useful for genetic improvement of low hydrocyanic acid sorghum-Sudan grass hybrid by marker-assisted breeding.

• 出版日期2016
• 单位内蒙古农业大学