Adsorption of Protein on Conducting Poly(methacryloyl pyrrole)

作者：Zhang Zhi Hong^*; Yan Li Jun; Liang Yan; Feng Xiao Zhong; Yan Fu Feng; Zheng Xian Jun; Wang Li Zhen

来源：Chemical Journal of Chinese Universities, 2010, 31(9): 1884-1889.

• Surface initiated atom transfer radical polymerization(SIATRP)
• Bovine serum albumin(BSA)
• Surfae plamson resonane spectrscopy(SPR)
• Polypyrrole

Poly(methacryloyl pyrrole) films were prepared by surface initiated atom transfer radical polymerization(SIATRP) onto the surface of silicon and Au. The chemical structure, the surface wettability, the surface morphology and the electrochemical property were investgated. The adsorption behavior of BSA on the polymer surface was measured in situ by surface plasmon resonance spectroscopy. The adsorption mechanism of BSA could be revealed based on the effects of buffer pH values and BSA concentration on the protein adsorption. The change in the chemical property after BSA immobilization was characterized by cyclic voltammetry. The morphologies of PMAP before and after BSA adsorption were investigated by atom force microscopy. This study could supply the base of the preparation of the regular, uniform and sensitive films which are used in the field of biosensors.

• 出版日期2010-9-10
• 单位郑州轻工业大学