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摘要
The objective of this study was to evaluate application of the essential oil of copaiba in controlling anthracnose in fruits of passion fruit and compare their fungicidal/fungistatic action in vitro with copaiba oleoresin. In vivo experiment, fruits were inoculated with a spore suspension of 10(6) conidia mL(-1) and 1% Tween 80, packed in polypropylene trays and placed in a dry incubator at temperature of 25 degrees C and 90% of relative humidity. After 24 hours of inoculation, essential oil was sprayed at the following concentrations: T1 = 0 mL L-1, T2 = 0.25 mL L-1, T3 = 0.5 ml L-1, T4 = 0.75 mL L-1, T5 = 1.0 mL L-1, and evaluated the mass loss of the fruit, anthracnose severity and number of lesions, both at six days. For the in vitro study, we used culture media potato dextrose agar (PDA) which, after being sterilized by autoclaving (120 degrees C), was added copaiba oleoresin and essential oil (0, 0.5, 1.0, 1.5 and 2.0 mL L-1). After cooling the PDA culture media was peaked to the center of the plate a mycelial disc of 12.5 mm diameter of Colletotrichum gloeosporioides and the plates incubated at 25 degrees C and 90% humidity. The measurement of mycelial growth was measured with the aid of analog caliper, after seven days of mycelial growth. The copaiba essential oil, in concentrations of 0.25 mL L-1 up to 1.0 mL L-1, was not effective in controlling post-harvest of the pathogen in vivo and in mass loss of passion fruits. The copaiba oils inhibited the growth of C. gloeosporioides in vitro more efficiently than the copaiba essential oil.
- 出版日期2014-6
