Analytical method for the determination of trace levels of steroid hormones and corticosteroids in soil, based on PLE/SPE/LC-MS/MS

作者：Gineys N; Giroud B; Vulliet E^*

来源：Analytical and Bioanalytical Chemistry, 2010, 397(6): 2295-2302.

DOI：10.1007/s00216-010-3787-4

• Soil
• Emerging pollutants
• Traces
• Analytical method
• Pressurized liquid extraction
• Liquid chromatography-tandem mass spectrometry

The aim of this study was to develop an efficient, sensitive and reliable analytical method for the determination of traces of steroid hormones (including oestrogen, androgens and progestagens) and corticosteroids in soil. A method of sample preparation involving pressurized liquid extraction (PLE) and solid-phase extraction (SPE) was developed for the determination of six steroids and five corticosteroids in soils, followed by analysis by liquid chromatography-tandem mass spectrometry. The conditions employed for PLE involved acetone/methanol (50:50) as the extracting solvent, a temperature of 80 A degrees C, two cycles and a static time of 5 min. The extraction was followed by a SPE clean-up based on a polymeric phase. With use of protocol, a residual matrix effect was, however, highlighted. The limit of detection in soil was 0.08-0.89 ng/g for steroids and 0.09-2.84 ng/g for corticosteroids.

• 出版日期2010-7