The Arabidopsis NSL2 negatively controls systemic acquired resistance via hypersensitive response

作者：Asada Yutaka^*; Yamamoto Masako; Tsutsui Tomokazu; Yamaguchi Junji

来源：Plant Biotechnology, 2011, 28(1): 9-15.

DOI：10.5511/plantbiotechnology.10.0913a

• Arabidopsis
• hypersensitive response
• lesion mimic mutant
• local inducible silencing
• Pseudomonas syringae pv. tomato DC3000

The Arabidopsis nsl2 (necrotic spotted lesion 2) mutant, which has been originally reported as the cad1 (constitutively activated cell death 1), shows a phenotype that mimics a hypersensitive response (HR)-like cell death. The NSL2 protein is suggested to negatively control the salicylic acid (SA)-mediated pathway of HR-like cell death in plant immunity. The induction of systemic acquired resistance (SAR) results in the induction of pathogenesis-related (PR) genes in systemic organs triggered by the local HR. In this report, we establish a NSL2 knockdown system in transgenic Arabidopsis based on constitutive or dexamethasone (DEX)-induced RNAi. The former showed a nsl2-like phenotype. In DEX-induced RNAi, localized knockdown resulted in the induction of PR1 gene expression and the restriction of bacterial growth in both DEX-treated and systemic leaves. These results indicate that NSL2 negatively controls SAR via HR.

• 出版日期2011