Antimicrobial photodynamic therapy using Indocyanine green and near-infrared diode laser in reducing Entrerococcus faecalis

作者：Beltes Charis^*; Sakkas Hercules; Economides Nikolaos; Papadopoulou Chrissanthy

来源：Photodiagnosis and Photodynamic Therapy, 2017, 17: 5-8.

DOI：10.1016/j.pdpdt.2016.10.012

• Antimicrobial photodynamic therapy
• Root-canal therapy
• Enterococcus faecalis

Background: The use of Antimicrobial Photodynamic Therapy (aPDT) has been suggested as an adjuvant method to eliminate facultative bacteria during root canal disinfection. The purpose of this preliminary in vitro study was to determine whether the light-activated antimicrobial agent, Indocyanine green (ICG), could be used as photosensitizer and kill Enterococcus faecalis strain under planktonic conditions when irradiated with near-infared (NIR) diode laser emitting in 810 nm wavelength.
Methods: Planktonic suspension containing Enterococcus faecalis strain was divided into nine experimental groups: (1) aPDT with ICG and laser (medium energy fluence), (2) aPDT with ICG and laser (high energy fluence), (3) only ICG without laser activation. (4) only laser emission without ICC (5)2.5% Sodium hypochiorite (NaOCl) as irrigant.(6) 2.5% NaOCl and aPDT with ICG and laser. (7)2.0% Chlorhexidine gluconate (CHX) as irrigant (8) No treatment (positive control). (9) No bacterial hiofilm growth (negative control). The samples were incubated for 7 days and colony-forming units (CFUs) were determined to evaluate bacterial viability.
Results: The microbiological test revealed that aPDT groups, regardless the overall power, showed significant lower mean logio CFU levels, than groups 3, 4 and 7 (p<0.01). The irrigation with 2.5% NaOCl and the combination of PDT and NaOCl achieved total elimination of bacteria.
Conclusion: These preliminary in vitro findings imply that the combination of ICG and NIR diode laser may be a novel supplement in aPDT and provide better disinfection during endodontic treatment.

• 出版日期2017-3