Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites

作者：Cui Xi Hua; Murthy Hosakatte Niranjana; Wu Chun Hua; Paek Kee Yoeup^*

来源：In Vitro Cellular and Developmental Biology - Plant, 2010, 46(5): 437-444.

DOI：10.1007/s11627-010-9310-y

• Adventitious root cultures
• Antioxidant activities
• Flavonoids
• Hypericum perforatum
• NH(4)(+)/NO(3)(-) ratio
• Phenols
• Shake flask culture

The present study investigated the effect of nitrogen source (NH (4) (+) ; NO (3) (-) ) at different concentrations on the accumulation of biomass and secondary metabolites in adventitious root cultures of Hypericum perforatum L. Cultures were initiated in shake flasks by using half-strength Murashige and Skoog (MS) medium with B5 vitamins, 1.0 mg l(-1) indole-3-butyric acid, 0.1 mg l(-1) kinetin, 3% (w/v) sucrose, and different ratios of ammonium and nitrate (0:30, 5:25, 10:20, 15:15, 20:10, 25:5, and 30:0 mM, using NH(4)Cl and KNO(3)). The cultures were maintained in darkness. The medium supplemented with 5:25 (mM) NH (4) (+) /NO (3) (-) resulted in the optimum accumulation of biomass and total phenols and flavonoids. The antioxidant potential of a methanolic extract, measured as the 1, 1-diphenyl-2-picrylhydrazyl and 2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities, of H. perforatum adventitious roots showed that antioxidant activity was high from root extracts that were grown on higher concentrations of NO (3) (-) nitrogen (15, 20, and 25 mM). Further, assessment of hydrogen peroxide (H(2)O(2)) and malondialdehyde content of the root extracts revealed that cultures supplemented with higher levels of NO (3) (-) nitrogen (15-30 mM) were under oxidative stress, which boosted the levels of secondary metabolites in the adventitious roots. These results suggest that optimal adventitious root biomass could be achieved with the supplementation of cultures with 5:25 ratios of MS nitrogen sources.

• 出版日期2010-10