Analysis of neovascularization after implanting porous silk fibroin films in rats

作者：Sun, Ziling^*; Bai, Lun

来源：Bio-medical Materials and Engineering, 2011, 21(4): 259-270.

DOI：10.3233/BME-2011-0674

• PSFFs
• angiogenesis
• endothelial cell
• VEGF
• HIF

The purpose of this paper is to explore neovascularization in the biomaterials implanted in vivo. Capillaries formation of dermis defect filled with porous silk fibroin films (PSFFs) implant were investigated in rat at different times. Vascular endothelial growth factor (VEGF), CD34 and hypoxia inducible factor-1 (HIF) were observed in inflammatory cells and fibroblast in PSFFs by day 1, but expression in HIF and VEGF decreased by day 7, otherwise VEGF expressed in vascular endothelial cell increased by day 15 and 25, respectively. VEGF, angiopoietin 1 (Ang-1) and integrins alpha v and integrins beta 3 in PSFFs were observed by Reverse transcription-polymerase chain reaction (RT-PCR). Polyvinyl alcohol (PVA) sponge was control. PSFFs have better neovascularization than that of PVA expression of VEGF, HIF and angiogenesis in immunohistochemical and mRNA level.

• 出版日期2011
• 单位苏州大学