Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

作者：Lien Truong Thi Ngoc; Lam Tran Dai^*; An Vu Thi Hong; Hoang Tran Vinh; Quang Duong Tuan; Khieu Dinh Quang; Tsukahara Toshifumi; Lee Young Hoon; Kim Jong Seung

来源：Talanta, 2010, 80(3): 1164-1169.

DOI：10.1016/j.talanta.2009.09.002

• MWCNTs-doped polypyrrole
• Electrochemical DNA biosensor
• Quartz crystal microbalance
• Electrochemical impedance spectroscopy

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R-ct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

• 出版日期2010-1-15