A time and cost-effective method for kappa-casein allele A and B genotyping of breeding bulls

作者：Flekna G^*; Zinovieva N; Muller M; Brem G

来源：Reproduction in Domestic Animals, 1997, 32(3): 171-173.

DOI：10.1111/j.1439-0531.1997.tb01277.x

The aim of this study was to establish a cost-effective and timesaving method for the routine diagnosis of kappa-casein genotypes of breeding bulls. DNA of 65 animals was isolated from semen by alkali lysis. The genotype was analysed by a single tube allele-specific (STAS) polymerase chain reaction (PCR). The results showed complete agreement with those of an established method, which is more costly.

• 出版日期1997-5