UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis

作者：White Mark D; Payne Karl A P; Fisher Karl; Marshall Stephen A; Parker David; Rattray Nicholas J W; Trivedi Drupad K; Goodacre Royston; Rigby Stephen E J; Scrutton Nigel S; Hay Sam; Leys David

来源：Nature, 2015, 522(7557): 502-+.

DOI：10.1038/nature14559

Ubiquinone (also known as coenzyme Q) is a ubiquitous lipid-soluble redox cofactor that is an essential component of electron transfer chains(1). Eleven genes have been implicated in bacterial ubiquinone biosynthesis, including ubiX and ubiD, which are responsible for decarboxylation of the 3-octaprenyl-4-hydroxy-benzoate precursor(2). Despite structural and biochemical characterization of UbiX as a flavin mononucleotide (FMN)-binding protein, no decarboxylase activity has been detected(3,4). Here we report that UbiX produces a novel flavin-derived cofactor required for the decarboxylase activity of UbiD(5). UbiX acts as a flavin prenyltransferase, linking a dimethylallyl moiety to the flavin N5 and C6 atoms. This adds a fourth non-aromatic ring to the flavin isoalloxazine group. In contrast to other prenyltransferases(6,7), UbiX is metal-independent and requires dimethylallyl-monophosphate as substrate. Kinetic crystallography reveals that the prenyltransferase mechanism of UbiX resembles that of the terpene synthases(8). The active site environment is dominated by pi systems, which assist phosphate-C1' bond breakage following FMN reduction, leading to formation of the N5-C1' bond. UbiX then acts as a chaperone for adduct reorientation, via transient carbocation species, leading ultimately to formation of the dimethylallyl C3'-C6 bond. Our findings establish the mechanism for formation of a new flavin-derived cofactor, extending both flavin and terpenoid biochemical repertoires.

• 出版日期2015-6-25