Assessment of tissue FoxP3(+), CD4(+) and CD8(+) T-cells in active and stable nonsegmental vitiligo

作者：Abdallah Marwa; Lotfi Ranya^*; Othman Wessam; Galal Riham

来源：International Journal of Dermatology, 2014, 53(8): 940-946.

DOI：10.1111/ijd.12160

Background The exact etiology of vitiligo remains obscure. Studies have indicated a role for cellular immunity in the pathogenesis of vitiligo. The aim of this study is to assess tissue FoxP3(+) natural regulatory T-cells (Tregs), as well as CD4(+) and CD8(+) T-cells in active vs. stable nonsegmental vitiligo. %26lt;br%26gt;Materials and methods Immunohistochemical double-staining for expression of CD4(+) and CD8(+) T-cells with immunostaining for expression of FoxP3 in lesional, marginal, and nonlesional skin of nonsegmental vitiligo was used to evaluate the abundance of Tregs among CD4(+) and CD8(+) T-cells in active and stable cases of vitiligo. %26lt;br%26gt;Results A significant increase in the number of CD4(+) and CD8(+) T-cells and a highly significant reduction in the number of FoxP3-expressing Tregs were detected in marginal skin in both stable and active vitiligo cases. FoxP3(+) cells were decreased in tissue of patients with vitiligo compared with healthy controls. The number of CD8(+) T-cells was increased in the epidermis and dermoepidermal junction (DEJ) in comparison with the number of CD4(+) T-cells. Tregs were mostly present at the DEJ. %26lt;br%26gt;Conclusion The reduction in the number of FoxP3(+) cells in the marginal skin suggests that this is the site where regulatory activity is needed to suppress the activity of helper and cytotoxic T-cells that are actively contributing to depigmentation.

• 出版日期2014-9