Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells

作者：Carneiro Zumira A; Biazzotto Juliana C; Alexiou Anamaria D P; Nikolaou Sofia^*

来源：Journal of Inorganic Biochemistry, 2014, 134: 36-38.

DOI：10.1016/j.jinorgbio.2014.01.012

• Triruthenium cluster
• Nitric oxide release
• Visible light irradiation
• Melanoma

vitro cytotoxicity study of the [Ru3O(CH3COO)(6)(4-pic)(2)(NO)]PF6 triruthenium nitrosyl cluster (compound 1, 4-pic = 4-methylpyridine) against B16F10 melanoma cell line was evaluated in the presence and absence of visible light irradiation. The nitrosyl cluster 1 showed a significant tumoricidal activity when irradiated at lambda = 532 nm, reducing cell viability up to 90% at a concentration of 62.5 mu m. However, cell death of 60% is also observed in the dark which can be assigned to the NO release mediated by a redox reaction of the cluster in cell medium. This possibility was confirmed by amperometric detection of NO after the addition of ascorbic acid to compound 1 in phosphate buffer. A control experiment was performed with the solvated cluster [Ru3O(CH3COO)(6)(4-pic)(2)(CH3OH)]PF6 (compound 2) and no significant lowering of cell viability was observed. These results suggest that the nitrosyl cluster acts as a pro-drug, delivering NO, which is the actual active species.

• 出版日期2014-5