Double strand break repair and gamma-H2AX formation in erythrocytes of medaka (Oryzias latipes) after gamma-irradiation

作者：Sayed Alaa El Din Hamid^*; Igarashi Kento; Watanabe Asaka Tomomi; Mitani Hiroshi

来源：Environmental Pollution, 2017, 224: 35-43.

DOI：10.1016/j.envpol.2016.11.050

• gamma-H2AX
• Erythrocytes
• DNA repair
• Fish
• Radiation

The study of the DNA damage response in erythrocytes after gamma-irradiation may provide evidence for its effectiveness as a biomarkers for genotoxic environmental stress. We previously reported various malformations in erythrocytes of medaka irradiated with10 Gy, but not in their micronuclei. In this study, we optimized an assay method for gamma-H2AX and double strand breaks in erythrocytes of adult medaka fish after 15 Gy of gamma-irradiation. The highest level of apoptosis and nuclear abnormalities, including in micronuclei, were recorded 4 h after gamma-irradiation, as was the highest level of gamma-H2AX foci in erythrocytes. These results suggest that recognition and repair processes occur as a response to DNA damage in erythrocytes in medaka.

• 出版日期2017-5